![]() ![]() The program identifies template structures and then automatically avoids them while designing HRMA primers, improving the PCR efficiency.īeacon Designer™ checks the Tm, GC % and other parameters of the primers designed. Reference gene or housekeeping genes supported for normalization.ĭesigns mutation flanking HRMA primers such that they generate the shortest possible amplicon, for mutation detection. Multiplex assays are supported in the TaqMan® design mode. ![]() Read about how TaqMan® probes work! Multiplex Real Time PCR Assay Designīeacon Designer™ uses innovative proprietary algorithms to design optimal primer-probe sets for single tube multiplex assays. You can also specify their placement and the LNA free region on each end of the probe to design LNA™ spiked TaqMan® probes. You can specify the number of LNA™ bases that you wish to have in the probe. LNA™ substituted TaqMan® probes are shorter probes and have LNA™ bases that impart higher stability.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |